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KMID : 1007520120210051463
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Food Science and Biotechnology
2012 Volume.21 No. 5 p.1463 ~ p.1468
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Purification and Characterization of Lysozyme from Filipino venus, Ruditapes philippinarum
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Kim Mi-Sook
Park Min-jeong
Jeong Yoon-Hwa
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Abstract
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Lysozyme from Filipino venus (Ruditapes philippinarum) was purified by ion-exchange and gel filtration chromatography. The purification fold and yield were 3,402 and 32.4%, respectively. The molecular weight was determined to be 13.4 kDa by SDS-PAGE. The specific activity of lysozyme was 3.76×105 units/mg protein with Micrococcus lysodeikticus as a substrate. The optimum temperature and pH of lysozyme were 75oC and 5.5, respectively. Lysozyme activity was decreased with about 45% after heat treatment for 30 min at 80oC, and completely inactivated at 100oC. It was activated by NaCl (10-70 mM), MgCl2, and CaCl2 (2-5 mM) whereas it was inhibited by ZnCl2 (2-30 mM).
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KEYWORD
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lysozyme, Filipino venus, purification, characterization
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